Type-II Major-Surface-Glycoprotein Family of Pneumocystis carinii under the Control of Novel Expression Elements
نویسندگان
چکیده
منابع مشابه
Type-II major-surface-glycoprotein family of Pneumocystis carinii.
Pneumocystis carinii are coated by abundant surface proteins, named MSGs for major surface glycoproteins, that undergo antigenic variation. There are many MSG genes on the chromosomes but the MSG expression site is restricted to a unique site called type-I UCS, and silent MSG genes can be translocated to the expression site by DNA recombination. We have reported previously the existence of alte...
متن کاملExpression of variants of the major surface glycoprotein of Pneumocystis carinii
Previously, we have shown that a multicopy family of related but unique genes encodes the major surface glycoprotein (MSG) of Pneumocystis carinii. To examine whether different members of this gene family are expressed by P. carinii, antisera were prepared against peptides whose sequences were determined from the deduced amino acid sequences of variants of rat-derived MSG. Immunohistochemical s...
متن کاملExpression, structure, and location of epitopes of the major surface glycoprotein of Pneumocystis carinii f. sp. carinii.
The major surface glycoprotein (MSG) of Pneumocystis carinii f. sp. carinii consists of a heterogeneous family of proteins that are encoded by approximately 100 unique genes. A genomic expression library was screened with a panel of MSG-specific monoclonal antibodies (MAbs) to identify conserved and rare epitopes. All of the antibodies reacted with epitopes that are encoded within the 5' end of...
متن کاملIdentification and characterization of novel variant major surface glycoprotein gene families in rat Pneumocystis carinii.
The major surface glycoprotein (MSG) is an abundant, immunodominant protein on the surface of the opportunistic pathogen Pneumocystis carinii. The current study identified two novel variant MSG (vMSG) gene families in rat P. carinii that are closely related to but distinct from MSG. These gene families encode proteins of approximately 90 kDa (v1MSG) and approximately 115 kDa (v2MSG). Compared w...
متن کاملMulti-gene family of major surface glycoproteins of Pneumocystis carinii: full-size cDNA cloning and expression.
The major surface glycoprotein (MSG) of Pneumocystis carinii plays a crucial role in the fatal pneumonia caused by this organism in AIDS patients. A cDNA encoding a full-length MSG polypeptide was isolated from a lambda library of rat-derived P. carinii cDNAs. The deduced MSG, referred to as the MSG5 subtype, is a 120,765-Da protein composed of 1,076 amino acids and contains an anchoring hydrop...
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ژورنال
عنوان ژورنال: DNA Research
سال: 1999
ISSN: 1340-2838
DOI: 10.1093/dnares/6.4.211